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Areas of specialization | |
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Laboratory/research office |
Molecular Cellular Genetics Laboratory
We are interested in the molecular mechanisms that stably repress the expression of transgenes, transposons, and some specific domestic genes. Our current study uses mainly single eukaryotic micro algae. We are also attempting to establish a system for stable and high expression of transgenes coding valid proteins. With such a system, micro unicellular alga can serve as a plant cell factory for the production of aimed proteins. |
Current reseach topics | |
Educational background |
: Bachelor
: Doctor |
Professional background |
1992-1994:
: : : |
Licenses | Academic societies |
Courses * Courses provided in English are shown with (E) mark
Undergraduate school ▼
Seminar 2
Seminar 3
Graduation Thesis
Gene Engineering
Biology Experiment
Genome Science
Molecular Biology
Seminar 3
Graduation Thesis
Gene Engineering
Biology Experiment
Genome Science
Molecular Biology
Graduate school ▼
Seminar 1
Seminar 2
Seminar 3
Seminar 4
Individual Work for Graduate
Advanced Seminar 1
Advanced Seminar 2
Current Topics in Life Science and Biotechnology
Seminar 2
Seminar 3
Seminar 4
Individual Work for Graduate
Advanced Seminar 1
Advanced Seminar 2
Current Topics in Life Science and Biotechnology
Research activities
Research papers ▼
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Authors: Sari Dewi Kurniasih, Tomohito Yamasaki, Fantao Kong, Sigeru Okada, Dwiyantari Widyaningrum, OHAMA Takeshi
Title: UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylationdependent and independent silencing systems
Journal: Plant Mol Biol, Vol. 92, No. 6, pp. 629-641, Springer
Year: 2016/10/19 -
Authors: Tomohito Yamasaki, Masayuki Onishi, Eun-Jeong Kim, Heriberto Cerutti, OHAMA Takeshi
Title: RNA-binding protein DUS16 plays an essential role in primary miRNA processing in the unicellular alga Chlamydomonas reinhardtii
Journal: Proceedings of the National Academy of Sciences (USA) , Vol. 113, No. 38, pp. 10720-10725, PNSA
Year: 2016/09/20 -
Authors: Miyasaka H, Ogata T, Tanaka S, OHAMA Takeshi, Kano S, Kazuhiro F, Hayashi S, Yamamoto S, Takahashi H, Matsuura H, Hirata K
Title: Is chloroplastic class IIA aldolase a marine enzyme?
Journal: The ISME Journal , Vol. 10, No. 11, pp. 2767-2772, Nature Publishing Group
Year: 2016/04/08 -
Authors: Takashi Kadono, Arisa Miyagawa-Yamaguchi, Nozomu Kira, Yuji Tomaru3, Takuma Okami, Takamichi Yoshimatsu, HOU Liyuan, OHAMA Takeshi, Kazunari Fukunaga, Masanori Okauchi5, Haruo Yamaguchi, Kohei Ohnishi, Angela Falciatore
Title: Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum
Journal: Scientific Reports, Vol. 5, No. 18708, pp. 1-13, Publishers of Nature
Year: 2015/12/22 -
Authors: Takashi Kadono, Nozomu Kira, Kengo Suzuki, Osamu Iwata, OHAMA Takeshi, Tomohiro Nishimura, Mai Akakabe, Masashi Tsuda, Masao Adachi
Title: Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum
Journal: Marine Drugs, Vol. 13, No. 8, pp. 5334-5357, MDPI
Year: 2015/08/18 -
Authors: KONG Fantao , Tomohito Yamasaki, Sari Dewi Kurniasih, HOU Liyuan , Xiaobo Li, Nina Ivanova, Shigeru Okada, Takeshi Ohama
Title: Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains
Journal: J. Bioscience and Bioengineering, Elsevjier
Year: 2015/02/03 -
Authors: HOU Liyuan , Hyunsun Park, Shigeru Okada, OHAMA, Takeshi
Title: Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments
Journal: Protoplasma, Vol. 251, No. 1, pp. 191-199, Springer
Year: 2014/01 -
Authors: KONG Fantao, Yamasaki, Tomohito, OHAMA, Takeshi
Title: Expression levels of domestic cDNA cassettes integrated in the nuclear genomes of various Chlamydomonas reinhardtii strains
Journal: J. Biosc.Bioeng.
Year: 2013/12 -
Authors: HOU Liyuan, Wang Pengyu, KONG Fantao, PARK,Hyun-Sun, KOBIRO, Kazuya, OHAMA, Takeshi
Title: Mesoporous TiO2 nanoparticles: a new material for biolistic bombardment
Journal: Phycol. Res, Vol. 61, pp. 58-60
Year: 2013/01/10 -
Authors: UCHIDA, Hidenobu, IKEUCHI, ERI, YAMASAKI, Tomohito, OHAMA, Takeshi
Title: The role of zinc finger protein in RNAi in a unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) in press
Journal: J Phycology
Year: 2012 -
Authors: T. Yamasaki, T. Ohama
Title: Involvement of Elongin C in the spread of repressive histone modifications
Journal: The Plant J.
Year: 2011/01/10 -
Authors: Yamasaki T, Ohama T
Title: Involvement of Elongin C in the spread of repressive histone modifications.
Journal: The Plant Journal, Vol. 65, pp. 51-61
Year: 2011 -
Authors: Park, HyunSun, OHAMA, Takeshi
Title: Biotransformation of a herb plant metabolite by a cell disruptant of Chlamydomonas reinhardtii
Journal: Biosci. Biotechnol. Biochem., Vol. 12, pp. 2803-2805
Year: 2009 -
Authors: OHAMA, Takeshi, Inagaki, Yuji, Bessho, Yoshitaka, Osawa, Syozo
Title: Evolving genetic code
Journal: Proceedings of the Japan Acad, Vol. 84, pp. 58-74
Year: 2008 -
Authors: Ikuta, K., Kawai, H., Müler, D.G., OHAMA, Takeshi
Title: Recurrent invasion of mitochondrial group II introns in specimens of Pylaiella littoralis (brown alga), collected worldwide
Journal: Current Gent, Vol. 53, pp. 207-216
Year: 2008 -
Authors: Yamasaki, Tomohito, Miyasaka, Hitoshi, OHAMA, Takeshi
Title: Unstable RNAi effect through methyl-CpG related transcriptional repression of inverted repeat transgene in Chlamydomonas reinhardtii.
Journal: Genetics, Vol. 180, pp. 1927-1944
Year: 2008 -
Authors: Yamasaki T, Miyasaka H, Ohama T
Title: Unstable RNAi effects through epigenetic silencing of an inverted repeat transgene in Chlamydomonas reinhardtii.
Journal: Genetics., Vol. 180, pp. 1927-1944
Year: 2008 -
Authors: Kurokawa, S, Tomohito Yamasaki, Komatsu, T., Watanabw, K., Ohama, T
Title: Degenerated recognition property of a mitochondrial Roming enzyme in the unicellular green alga chlamydomonas smithii
Journal: Plant Molecular Biology, Vol. 62, pp. 141-150
Year: 2006 -
Authors: Kurokawa S, Yamasaki T, Komatsu T, Watanabe KI, Ohama T
Title: Degenerated recognition property of a mitochondrial homing enzyme in the unicellular green alga Chlamydomonas smithii.
Journal: Plant Mol Biol., Vol. 62, pp. 141-150
Year: 2006 -
Authors: Yamasaki, T, Kurokawa, S., Watanabe, K., Ikuta, K., Ohama, T
Title: Shared moleculag charactrics of success fully tranoformed mitochondrial genomes in Chlamydomonas reinhaidtii
Journal: Plant Mol. Biol, No. 58, pp. 515-527
Year: 2005 -
Authors: Yamasaki T, Kurokawa S, Watanabe KI, Ikuta K, Ohama T
Title: Shared molecular characteristics of successfully transformed mitochondrial genomes in Chlamydomonas reinhardtii.
Journal: Plant Mol Biol., Vol. 58, pp. 515-527
Year: 2005 -
Authors: Kurokawa, S, Bessho, Y., Watanabe, K, Ohama, T
Title: Adaptation of intronic homing endonuclease for successful hurizontal transmission
Journal: FEBS J, No. 272, pp. 2487-2496
Year: 2005 -
Authors: Watanabe, I.K., Ohama, T
Title: Regular spliceosomal introns are invasive in Chlamydomonas reinhardtii : 15 introns in the recently relocated mitochondrial cox2 and cox3 genes
Journal: J. Mol. Evol, Vol. 53, No. 4-5, pp. 333-339
Year: 2001 -
Authors: Ehara, M., Watanabe, I.K., Ohama, T
Title: Distribution of cognates of group II introns detected in mitochondrial cox1 genes of a diatom and a haptophyte
Journal: Gene, No. 256, pp. 157-167
Year: 2000 -
Authors: Ehara, M, Inagaki, Y., Watanabe, I.K., Ohama, T
Title: Phylogenetic analysis of diatom coxI genes and implications of fluctuating GC content on mitochondrial genetic code evolution
Journal: Current Genetics, No. 37, pp. 29-33
Year: 2000 -
Authors: Inagaki, Y., Dacks, J., Doolittle, W.F., Watanabe, K., Ohama, T
Title: Evolutionary relationship between dinoflagellates bearing obligate diatom endosymbionts : insight into tertiary endosymbiosis
Journal: Int. J. Sys. Evol. Microbiol, No. 50, pp. 2075-2081
Year: 2000 -
Authors: Hayashi-Ishimaru, Ehara, M, Ohama, T, Inagaki, Y
Title: Adeviant mitochondrial genetic code in prymnesiophytes (yellow-algae) : UGA codon for tryptophan.
Journal: Current Genetics, No. 32, pp. 296-299
Year: 1997 -
Authors: Ohama, T, Suzuki, T., Mori, M, Osawa, S., Ueda, T, Watanabe, K, Nakase, T
Title: Non-univrsal decoding of the leucine codon CUG in ceveral Candida species.,
Journal: Nucl. Acids Res., No. 21, pp. 4039-4045
Year: 1993 -
Authors: Jukes, T. H, Bessho, Y, Ohama, T, Osawa, S
Title: Release factors and genetic code ( Scientific Correspondence)
Journal: Nature, No. 352, p. 575
Year: 1991 -
Authors: Osawa, S, Muto, A, Ohama, T, Andachi, Y, Tanaka, R, Yamano, F
Title: Procaryotic genetic code. Genetic code 1990 EXPERIENTIA
Journal: , No. 46, pp. 1097-1106
Year: 1990
Keynote lectures ▼
- The RNA-binding protein DUS16 plays an essential role in primary miRNA processing in Chlamydomnas , The international conference on the Cell and Molucular biology of Chlamydomas, Kyoto, Japan, 2016/06/26
- , 2015/09/06
- , 2015/09/03
Professor OHAMA Takeshi
Material and Life Systems Engineering Course, Department of Engineering, Graduate School of Enginering
Environmental Systems Engineering Course, Department of Engineering, Graduate School of Enginering