Faculty Members


Professor OHAMA Takeshi

Year of birth 1956 Gender Male
Affiliation School of Environmental Science and Engineering
Material and Life Systems Engineering Course, Department of Engineering, Graduate School of Enginering
Environmental Systems Engineering Course, Department of Engineering, Graduate School of Enginering
Job titles
Personal web site

Areas of specialization
Laboratory/research office Molecular Cellular Genetics Laboratory
We are interested in the molecular mechanisms that stably repress the expression of transgenes, transposons, and some specific domestic genes. Our current study uses mainly single eukaryotic micro algae. We are also attempting to establish a system for stable and high expression  of transgenes coding valid proteins. With such a system, micro unicellular alga can serve as a plant cell factory for the production of aimed proteins.
Current reseach topics
Educational background : Bachelor
: Doctor
Professional background 1992-1994: 

Academic societies

Courses  * Courses provided in English are shown with (E) mark

Undergraduate school ▼

Seminar 2
Seminar 3
Graduation Thesis
Gene Engineering
Biology Experiment
Genome Science
Molecular Biology

Graduate school ▼

Seminar 1
Seminar 2
Seminar 3
Seminar 4
Individual Work for Graduate
Advanced Seminar 1
Advanced Seminar 2
Current Topics in Life Science and Biotechnology

Research activities

Research papers ▼

  1. Authors: Sari Dewi Kurniasih, Tomohito Yamasaki, Fantao Kong, Sigeru Okada, Dwiyantari Widyaningrum, OHAMA Takeshi
    Title: UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylationdependent and independent silencing systems
    Journal: Plant Mol Biol, Vol. 92, No. 6, pp. 629-641, Springer
    Year: 2016/10/19
  2. Authors: Tomohito Yamasaki, Masayuki Onishi, Eun-Jeong Kim, Heriberto Cerutti, OHAMA Takeshi
    Title: RNA-binding protein DUS16 plays an essential role in primary miRNA processing in the unicellular alga Chlamydomonas reinhardtii
    Journal:  Proceedings of the National Academy of Sciences (USA) , Vol. 113, No. 38, pp. 10720-10725, PNSA
    Year: 2016/09/20
  3. Authors: Miyasaka H, Ogata T, Tanaka S, OHAMA Takeshi, Kano S, Kazuhiro F, Hayashi S, Yamamoto S, Takahashi H, Matsuura H, Hirata K
    Title: Is chloroplastic class IIA aldolase a marine enzyme?
    Journal: The ISME Journal , Vol. 10, No. 11, pp. 2767-2772, Nature Publishing Group
    Year: 2016/04/08
  4. Authors: Takashi Kadono, Arisa Miyagawa-Yamaguchi, Nozomu Kira, Yuji Tomaru3, Takuma Okami, Takamichi Yoshimatsu, HOU Liyuan, OHAMA Takeshi, Kazunari Fukunaga, Masanori Okauchi5, Haruo Yamaguchi, Kohei Ohnishi, Angela Falciatore
    Title: Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum
    Journal: Scientific Reports, Vol. 5, No. 18708, pp. 1-13, Publishers of Nature
    Year: 2015/12/22
  5. Authors: Takashi Kadono, Nozomu Kira, Kengo Suzuki, Osamu Iwata, OHAMA Takeshi, Tomohiro Nishimura, Mai Akakabe, Masashi Tsuda, Masao Adachi
    Title: Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum
    Journal: Marine Drugs, Vol. 13, No. 8, pp. 5334-5357, MDPI
    Year: 2015/08/18
  6. Authors: KONG Fantao , Tomohito Yamasaki, Sari Dewi Kurniasih, HOU Liyuan , Xiaobo Li, Nina Ivanova, Shigeru Okada, Takeshi Ohama
    Title: Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains
    Journal: J. Bioscience and Bioengineering, Elsevjier
    Year: 2015/02/03
  7. Authors: HOU Liyuan , Hyunsun Park, Shigeru Okada, OHAMA, Takeshi
    Title: Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments
    Journal: Protoplasma, Vol. 251, No. 1, pp. 191-199, Springer
    Year: 2014/01
  8. Authors: KONG Fantao, Yamasaki, Tomohito, OHAMA, Takeshi
    Title: Expression levels of domestic cDNA cassettes integrated in the nuclear genomes of various Chlamydomonas reinhardtii strains
    Journal: J. Biosc.Bioeng.
    Year: 2013/12
  9. Authors: HOU Liyuan, Wang Pengyu, KONG Fantao, PARK,Hyun-Sun, KOBIRO, Kazuya, OHAMA, Takeshi
    Title: Mesoporous TiO2 nanoparticles: a new material for biolistic bombardment
    Journal: Phycol. Res, Vol. 61, pp. 58-60
    Year: 2013/01/10
  10. Authors: UCHIDA, Hidenobu, IKEUCHI, ERI, YAMASAKI, Tomohito, OHAMA, Takeshi
    Title: The role of zinc finger protein in RNAi in a unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) in press
    Journal: J Phycology
    Year: 2012
  11. Authors: T. Yamasaki, T. Ohama
    Title: Involvement of Elongin C in the spread of repressive histone modifications
    Journal: The Plant J.
    Year: 2011/01/10
  12. Authors: Yamasaki T, Ohama T
    Title: Involvement of Elongin C in the spread of repressive histone modifications.
    Journal: The Plant Journal, Vol. 65, pp. 51-61
    Year: 2011
  13. Authors: Park, HyunSun, OHAMA, Takeshi
    Title: Biotransformation of a herb plant metabolite by a cell disruptant of Chlamydomonas reinhardtii
    Journal: Biosci. Biotechnol. Biochem., Vol. 12, pp. 2803-2805
    Year: 2009
  14. Authors: OHAMA, Takeshi, Inagaki, Yuji, Bessho, Yoshitaka, Osawa, Syozo
    Title: Evolving genetic code
    Journal: Proceedings of the Japan Acad, Vol. 84, pp. 58-74
    Year: 2008
  15. Authors: Ikuta, K., Kawai, H., Müler, D.G., OHAMA, Takeshi
    Title: Recurrent invasion of mitochondrial group II introns in specimens of Pylaiella littoralis (brown alga), collected worldwide
    Journal: Current Gent, Vol. 53, pp. 207-216
    Year: 2008
  16. Authors: Yamasaki, Tomohito, Miyasaka, Hitoshi, OHAMA, Takeshi
    Title: Unstable RNAi effect through methyl-CpG related transcriptional repression of inverted repeat transgene in Chlamydomonas reinhardtii.
    Journal: Genetics, Vol. 180, pp. 1927-1944
    Year: 2008
  17. Authors: Yamasaki T, Miyasaka H, Ohama T
    Title: Unstable RNAi effects through epigenetic silencing of an inverted repeat transgene in Chlamydomonas reinhardtii.
    Journal: Genetics., Vol. 180, pp. 1927-1944
    Year: 2008
  18. Authors: Kurokawa, S, Tomohito Yamasaki, Komatsu, T., Watanabw, K., Ohama, T
    Title: Degenerated recognition property of a mitochondrial Roming enzyme in the unicellular green alga chlamydomonas smithii
    Journal: Plant Molecular Biology, Vol. 62, pp. 141-150
    Year: 2006
  19. Authors: Kurokawa S, Yamasaki T, Komatsu T, Watanabe KI, Ohama T
    Title: Degenerated recognition property of a mitochondrial homing enzyme in the unicellular green alga Chlamydomonas smithii.
    Journal: Plant Mol Biol., Vol. 62, pp. 141-150
    Year: 2006
  20. Authors: Yamasaki, T, Kurokawa, S., Watanabe, K., Ikuta, K., Ohama, T
    Title: Shared moleculag charactrics of success fully tranoformed mitochondrial genomes in Chlamydomonas reinhaidtii
    Journal: Plant Mol. Biol, No. 58, pp. 515-527
    Year: 2005
  21. Authors: Yamasaki T, Kurokawa S, Watanabe KI, Ikuta K, Ohama T
    Title: Shared molecular characteristics of successfully transformed mitochondrial genomes in Chlamydomonas reinhardtii.
    Journal: Plant Mol Biol., Vol. 58, pp. 515-527
    Year: 2005
  22. Authors: Kurokawa, S, Bessho, Y., Watanabe, K, Ohama, T
    Title: Adaptation of intronic homing endonuclease for successful hurizontal transmission
    Journal: FEBS J, No. 272, pp. 2487-2496
    Year: 2005
  23. Authors: Watanabe, I.K., Ohama, T
    Title: Regular spliceosomal introns are invasive in Chlamydomonas reinhardtii : 15 introns in the recently relocated mitochondrial cox2 and cox3 genes
    Journal: J. Mol. Evol, Vol. 53, No. 4-5, pp. 333-339
    Year: 2001
  24. Authors: Ehara, M., Watanabe, I.K., Ohama, T
    Title: Distribution of cognates of group II introns detected in mitochondrial cox1 genes of a diatom and a haptophyte
    Journal: Gene, No. 256, pp. 157-167
    Year: 2000
  25. Authors: Ehara, M, Inagaki, Y., Watanabe, I.K., Ohama, T
    Title: Phylogenetic analysis of diatom coxI genes and implications of fluctuating GC content on mitochondrial genetic code evolution
    Journal: Current Genetics, No. 37, pp. 29-33
    Year: 2000
  26. Authors: Inagaki, Y., Dacks, J., Doolittle, W.F., Watanabe, K., Ohama, T
    Title: Evolutionary relationship between dinoflagellates bearing obligate diatom endosymbionts : insight into tertiary endosymbiosis
    Journal: Int. J. Sys. Evol. Microbiol, No. 50, pp. 2075-2081
    Year: 2000
  27. Authors: Hayashi-Ishimaru, Ehara, M, Ohama, T, Inagaki, Y
    Title: Adeviant mitochondrial genetic code in prymnesiophytes (yellow-algae) : UGA codon for tryptophan.
    Journal: Current Genetics, No. 32, pp. 296-299
    Year: 1997
  28. Authors: Ohama, T, Suzuki, T., Mori, M, Osawa, S., Ueda, T, Watanabe, K, Nakase, T
    Title: Non-univrsal decoding of the leucine codon CUG in ceveral Candida species.,
    Journal: Nucl. Acids Res., No. 21, pp. 4039-4045
    Year: 1993
  29. Authors: Jukes, T. H, Bessho, Y, Ohama, T, Osawa, S
    Title: Release factors and genetic code ( Scientific Correspondence)
    Journal: Nature, No. 352, p. 575
    Year: 1991
  30. Authors: Osawa, S, Muto, A, Ohama, T, Andachi, Y, Tanaka, R, Yamano, F
    Title: Procaryotic genetic code. Genetic code 1990 EXPERIENTIA
    Journal: , No. 46, pp. 1097-1106
    Year: 1990

Keynote lectures ▼

  1. The RNA-binding protein DUS16 plays an essential role in primary miRNA processing in Chlamydomnas , The international conference on the Cell and Molucular biology of Chlamydomas, Kyoto, Japan, 2016/06/26
  2. , 2015/09/06
  3. , 2015/09/03

Invited lectures ▼

Notable projects ▼

Awards ▼

Patents ▼

Grants-in-Aid for Scientific Research from the Japanese government ▼

Competitive research funds ▼

Social activities

Committee roles ▼

Published books ▼