Faculty Members

Professor
OHAMA, Takeshi

Year of birth: 1956
Gender: Male
Affiliation:

- School of Engineering Science
- School of Environmental Science and Engineering
- Life Science and Technology, Department of Engineering, Graduate School of Enginering

Areas of specialization
Laboratory/research office	Laboratory of Molecular Cellular Genetics We are interested in the molecular mechanisms that stably repress the expression of transgenes, transposons, and some specific domestic genes. Single eukaryotic micro algae are mainly used for this study. We are also challenging to establish the system that is useful for stable and high expression of transgenes coding valid proteins. Through such a system, micro unicellular alga can be a plant cell factory for the production of aimed proteins.
Current research topics
Educational background
Professional background
Licenses
Academic societies

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Courses

* Courses provided in English are shown with (E) mark

Undergraduate school	Seminar 2 Seminar 3 Graduation Thesis Gene Engineering Biology Experiment Genome Science Plant functional morphology Dynamics of cell function
Graduate school	Seminar 1 Seminar 2 Seminar 3 Seminar 4 Individual Work for Graduate Advanced Seminar 1 Advanced Seminar 2 Molecular genetics of eukaryotes

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Research activities

Research papers	Authors: Han Lu, Keqing Liu, Hao Zhang, Xi Xie, Yunlong Ge, Zhanyou Chi, Song Xu, Fantao Kong, Takeshi Ohama Title: Enhanced triacyclglycerols and starch synthesis in Chlamydomonas stimulated by the engineered biodegradable nanoparticles Journal: Applied Microbiology and Biotechnology, Springer Year: 2023 Authors: Ayat Al-Azab, Yuichi Aoki, Fean Sarian, Yuki Sori, Dwiyantari Widyaningrum, Tomohito Yamasaki, Fantao Kong, Takeshi Ohama Title: Chronological transcriptome changes induced by exposure to cyanoacrylate resin nanoparticles in Chlamydomonas reinhardtii with a focus on ROS development and cell wall lysis-related genes Journal: Algal Res., Elsevier Year: 2022 Authors: Fean Davisunjaya Sarian, Kazuki Ando, Shota Tsurumi, Miyashita R, Ute K, Takeshi Ohama Title: Evaluation of the Growth-Inhibitory Spectrum of Three Types of Cyanoacrylate Nanoparticles on Gram-Positive and Gram-Negative Bacteria. Journal: Membranes, MDPI Year: 2022 Authors: Ayat Jabr Salem Al-azab, Dwiyantari Widyaningrum, Haruna Hirakawa, Yasuko Hayashi, Satoshi Tanaka, Takeshi Ohama Title: A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae Journal: Algal Reserch, Vol. 54, No. 1, pp. 1-9, ELSEVIER Year: 2021 Authors: Dwiyantari Widyaningrum, Daisuke Iida, Yuma Tanabe, Yasuko Hayashi, Sari Dewi Kurniasih Indrawan, Takeshi Ohama Title: Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (chlorophyceae) following exposure to acrylic resin nanoparticles Journal: J. Phycology Year: 2018 Authors: Sari Dewi Kurniasih, Tomohito Yamasaki, Fantao Kong, Sigeru Okada, Dwiyantari Widyaningrum, OHAMA Takeshi Title: UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylationdependent and independent silencing systems Journal: Plant Mol Biol, Vol. 92, No. 6, pp. 629-641, Springer Year: 2016 Authors: Tomohito Yamasaki, Masayuki Onishi, Eun-Jeong Kim, Heriberto Cerutti, OHAMA Takeshi Title: RNA-binding protein DUS16 plays an essential role in primary miRNA processing in the unicellular alga Chlamydomonas reinhardtii Journal: Proceedings of the National Academy of Sciences (USA) , Vol. 113, No. 38, pp. 10720-10725, PNSA Year: 2016 Authors: Miyasaka H, Ogata T, Tanaka S, OHAMA Takeshi, Kano S, Kazuhiro F, Hayashi S, Yamamoto S, Takahashi H, Matsuura H, Hirata K Title: Is chloroplastic class IIA aldolase a marine enzyme? Journal: The ISME Journal , Vol. 10, No. 11, pp. 2767-2772, Nature Publishing Group Year: 2016 Authors: Takashi Kadono, Arisa Miyagawa-Yamaguchi, Nozomu Kira, Yuji Tomaru3, Takuma Okami, Takamichi Yoshimatsu, HOU Liyuan, OHAMA Takeshi, Kazunari Fukunaga, Masanori Okauchi5, Haruo Yamaguchi, Kohei Ohnishi, Angela Falciatore Title: Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum Journal: Scientific Reports, Vol. 5, No. 18708, pp. 1-13, Publishers of Nature Year: 2015 Authors: Takashi Kadono, Nozomu Kira, Kengo Suzuki, Osamu Iwata, OHAMA Takeshi, Tomohiro Nishimura, Mai Akakabe, Masashi Tsuda, Masao Adachi Title: Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum Journal: Marine Drugs, Vol. 13, No. 8, pp. 5334-5357, MDPI Year: 2015 Authors: KONG Fantao , Tomohito Yamasaki, Sari Dewi Kurniasih, HOU Liyuan , Xiaobo Li, Nina Ivanova, Shigeru Okada, Takeshi Ohama Title: Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains Journal: J. Bioscience and Bioengineering, Elsevjier Year: 2015 Authors: HOU Liyuan , Hyunsun Park, Shigeru Okada, OHAMA Takeshi Title: Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments Journal: Protoplasma, Vol. 251, No. 1, pp. 191-199, Springer Year: 2014 Authors: KONG Fantao, Yamasaki Tomohito, OHAMA Takeshi Title: Expression levels of domestic cDNA cassettes integrated in the nuclear genomes of various Chlamydomonas reinhardtii strains Journal: J. Biosc.Bioeng. Year: 2013 Authors: HOU Liyuan, Wang Pengyu, KONG Fantao, PARKHyun-Sun, KOBIRO Kazuya, OHAMA Takeshi Title: Mesoporous TiO2 nanoparticles: a new material for biolistic bombardment Journal: Phycol. Res, Vol. 61, pp. 58-60 Year: 2013 Authors: UCHIDA Hidenobu, IKEUCHI ERI, YAMASAKI Tomohito, OHAMA Takeshi Title: The role of zinc finger protein in RNAi in a unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) in press Journal: J Phycology Year: 2012 Authors: T. Yamasaki, T. Ohama Title: Involvement of Elongin C in the spread of repressive histone modifications Journal: The Plant J. Year: 2011 Authors: Yamasaki T, Ohama T Title: Involvement of Elongin C in the spread of repressive histone modifications. Journal: The Plant Journal, Vol. 65, pp. 51-61 Year: 2011 Authors: Park HyunSun, OHAMA Takeshi Title: Biotransformation of a herb plant metabolite by a cell disruptant of Chlamydomonas reinhardtii Journal: Biosci. Biotechnol. Biochem., Vol. 12, pp. 2803-2805 Year: 2009 Authors: OHAMA Takeshi, Inagaki Yuji, Bessho Yoshitaka, Osawa Syozo Title: Evolving genetic code Journal: Proceedings of the Japan Acad, Vol. 84, pp. 58-74 Year: 2008 Authors: Yamasaki Tomohito, Miyasaka Hitoshi, OHAMA Takeshi Title: Unstable RNAi effect through methyl-CpG related transcriptional repression of inverted repeat transgene in Chlamydomonas reinhardtii. Journal: Genetics, Vol. 180, pp. 1927-1944 Year: 2008 Authors: Yamasaki T, Miyasaka H, Ohama T Title: Unstable RNAi effects through epigenetic silencing of an inverted repeat transgene in Chlamydomonas reinhardtii. Journal: Genetics., Vol. 180, pp. 1927-1944 Year: 2008 Authors: Ikuta K., Kawai H., Müler D.G., OHAMA Takeshi Title: Recurrent invasion of mitochondrial group II introns in specimens of Pylaiella littoralis (brown alga), collected worldwide Journal: Current Gent, Vol. 53, pp. 207-216 Year: 2008 Authors: Kurokawa S, Yamasaki T, Komatsu T, Watanabe KI, Ohama T Title: Degenerated recognition property of a mitochondrial homing enzyme in the unicellular green alga Chlamydomonas smithii. Journal: Plant Mol Biol., Vol. 62, pp. 141-150 Year: 2006 Authors: Kurokawa S, Tomohito Yamasaki, Komatsu T., Watanabw K., Ohama T Title: Degenerated recognition property of a mitochondrial Roming enzyme in the unicellular green alga chlamydomonas smithii Journal: Plant Molecular Biology, Vol. 62, pp. 141-150 Year: 2006 Authors: Yamasaki T, Kurokawa S, Watanabe KI, Ikuta K, Ohama T Title: Shared molecular characteristics of successfully transformed mitochondrial genomes in Chlamydomonas reinhardtii. Journal: Plant Mol Biol., Vol. 58, pp. 515-527 Year: 2005 Authors: Yamasaki T, Kurokawa S., Watanabe K., Ikuta K., Ohama T Title: Shared moleculag charactrics of success fully tranoformed mitochondrial genomes in Chlamydomonas reinhaidtii Journal: Plant Mol. Biol, No. 58, pp. 515-527 Year: 2005 Authors: Kurokawa S, Bessho Y., Watanabe K, Ohama T Title: Adaptation of intronic homing endonuclease for successful hurizontal transmission Journal: FEBS J, No. 272, pp. 2487-2496 Year: 2005 Authors: Watanabe I.K., Ohama T Title: Regular spliceosomal introns are invasive in Chlamydomonas reinhardtii : 15 introns in the recently relocated mitochondrial cox2 and cox3 genes Journal: J. Mol. Evol, Vol. 53, No. 4-5, pp. 333-339 Year: 2001 Authors: Ehara M, Inagaki Y., Watanabe I.K., Ohama T Title: Phylogenetic analysis of diatom coxI genes and implications of fluctuating GC content on mitochondrial genetic code evolution Journal: Current Genetics, No. 37, pp. 29-33 Year: 2000 Authors: Inagaki Y., Dacks J., Doolittle W.F., Watanabe K., Ohama T Title: Evolutionary relationship between dinoflagellates bearing obligate diatom endosymbionts : insight into tertiary endosymbiosis Journal: Int. J. Sys. Evol. Microbiol, No. 50, pp. 2075-2081 Year: 2000 Authors: Ehara M., Watanabe I.K., Ohama T Title: Distribution of cognates of group II introns detected in mitochondrial cox1 genes of a diatom and a haptophyte Journal: Gene, No. 256, pp. 157-167 Year: 2000 Authors: Hayashi-Ishimaru, Ehara M, Ohama T, Inagaki Y Title: Adeviant mitochondrial genetic code in prymnesiophytes (yellow-algae) : UGA codon for tryptophan. Journal: Current Genetics, No. 32, pp. 296-299 Year: 1997 Authors: Ohama T, Suzuki T., Mori M, Osawa S., Ueda T, Watanabe K, Nakase T Title: Non-univrsal decoding of the leucine codon CUG in ceveral Candida species., Journal: Nucl. Acids Res., No. 21, pp. 4039-4045 Year: 1993 Authors: Jukes T. H, Bessho Y, Ohama T, Osawa S Title: Release factors and genetic code ( Scientific Correspondence) Journal: Nature, No. 352, p. 575 Year: 1991 Authors: Osawa S, Muto A, Ohama T, Andachi Y, Tanaka R, Yamano F Title: Procaryotic genetic code. Genetic code 1990 EXPERIENTIA Journal: No. 46, pp. 1097-1106 Year: 1990
Keynote lectures	Potential use of isobutyl-cyanoacrylate nanopartticle for inducing rapid cell wall disruption in Chlorella vurgaris , 18th INITERNATIONAL CONFERENCE ON THE CELL AND MOLECULAR BIOLOGY OF CHLAMYDOMONAS, Washington DC. USA, 2018 The RNA-binding protein DUS16 plays an essential role in primary miRNA processing in Chlamydomnas , The international conference on the Cell and Molucular biology of Chlamydomas, Kyoto, Japan, 2016
Grants-in-Aid for Scientific Research from the Japanese government	Project title: Analyses of abnormal physiological responses induced by stable resin nanoparticles and its species spectrum Category: Grant-in-Aid for Scientific Research (C) Project number: 19K12422 Project period: 2019-2021 Total budget amount: 4,290,000 yen Keywords:

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Social activities

Committee roles	Molecular Genetic Tools and Emerging Synthetic Biology Strategies to Increase Cellular Oil Content in Chlamydomonas reinhardtii, 2019

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