Faculty Members

LANGUAGE ≫ Japanese

OHAMA, Takeshi

  • Year of birth: 1956
  • Gender: Male
  • Affiliation:
    - School of Engineering Science
    - School of Environmental Science and Engineering
    - Life Science and Technology, Department of Engineering, Graduate School of Enginering
Areas of specialization
Laboratory/research office Laboratory of Molecular Cellular Genetics
We are interested in the molecular mechanisms that stably repress the expression of transgenes, transposons, and some specific domestic genes. Single eukaryotic micro algae are mainly used for this study. We are also challenging to establish the system that is useful for stable and high expression of transgenes coding valid proteins. Through such a system, micro unicellular alga can be a plant cell factory for the production of aimed proteins.
Current research topics
Educational background
Professional background
Academic societies

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* Courses provided in English are shown with (E) mark

Undergraduate school
Graduate school

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Research activities

Research papers
  1. Authors: Fean Davisunjaya Sarian, Takeshi Ohama
    Title: Comparable antibacterial effects and action mechanisms of ethyl cyanoacrylate nanoparticles on Bacillus subtilis and Escherichia coli evaluated by transcriptome and morphological changes
    Journal: Environmental Science: Nano, Vol. 10, pp. 1932-1941
    Year: 2023
  2. Authors: Fantao Kong, Mengjie Li, Keqing Liu, Yunlong Ge, Tomohito Yamasaki, Audrey Beyly-Adriano, Takeshi Ohama, Yonghua Li-Beisson
    Title: Efficient approaches for nuclear transgene stacking in the unicellular green microalga Chlamydomonas reinhardtii
    Journal: Algal Research, Vol. 71
    Year: 2023
  3. Authors: Han Lu, Keqing Liu, Hao Zhang, Xi Xie, Yunlong Ge, Zhanyou Chi, Song Xu, Fantao Kong, Takeshi Ohama
    Title: Enhanced triacyclglycerols and starch synthesis in Chlamydomonas stimulated by the engineered biodegradable nanoparticles
    Journal: Applied Microbiology and Biotechnology, Springer
    Year: 2023
  4. Authors: Ayat Al-Azab, Yuichi Aoki, Fean Sarian, Yuki Sori, Dwiyantari Widyaningrum, Tomohito Yamasaki, Fantao Kong, Takeshi Ohama
    Title: Chronological transcriptome changes induced by exposure to cyanoacrylate resin nanoparticles in Chlamydomonas reinhardtii with a focus on ROS development and cell wall lysis-related genes
    Journal: Algal Res., Elsevier
    Year: 2022
  5. Authors: Fean Davisunjaya Sarian, Kazuki Ando, Shota Tsurumi, Miyashita R, Ute K, Takeshi Ohama
    Title: Evaluation of the Growth-Inhibitory Spectrum of Three Types of Cyanoacrylate Nanoparticles on Gram-Positive and Gram-Negative Bacteria.
    Journal: Membranes, MDPI
    Year: 2022
  6. Authors: Ayat Jabr Salem Al-azab, Dwiyantari Widyaningrum, Haruna Hirakawa, Yasuko Hayashi, Satoshi Tanaka, Takeshi Ohama
    Title: A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae
    Journal: Algal Reserch, Vol. 54, No. 1, pp. 1-9, ELSEVIER
    Year: 2021
  7. Authors: Dwiyantari Widyaningrum, Daisuke Iida, Yuma Tanabe, Yasuko Hayashi, Sari Dewi Kurniasih Indrawan, Takeshi Ohama
    Title: Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (chlorophyceae) following exposure to acrylic resin nanoparticles
    Journal: J. Phycology
    Year: 2018
  8. Authors: Sari Dewi Kurniasih, Tomohito Yamasaki, Fantao Kong, Sigeru Okada, Dwiyantari Widyaningrum, OHAMA Takeshi
    Title: UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylationdependent and independent silencing systems
    Journal: Plant Mol Biol, Vol. 92, No. 6, pp. 629-641, Springer
    Year: 2016
  9. Authors: Tomohito Yamasaki, Masayuki Onishi, Eun-Jeong Kim, Heriberto Cerutti, OHAMA Takeshi
    Title: RNA-binding protein DUS16 plays an essential role in primary miRNA processing in the unicellular alga Chlamydomonas reinhardtii
    Journal:  Proceedings of the National Academy of Sciences (USA) , Vol. 113, No. 38, pp. 10720-10725, PNSA
    Year: 2016
  10. Authors: Miyasaka H, Ogata T, Tanaka S, OHAMA Takeshi, Kano S, Kazuhiro F, Hayashi S, Yamamoto S, Takahashi H, Matsuura H, Hirata K
    Title: Is chloroplastic class IIA aldolase a marine enzyme?
    Journal: The ISME Journal , Vol. 10, No. 11, pp. 2767-2772, Nature Publishing Group
    Year: 2016
  11. Authors: Takashi Kadono, Arisa Miyagawa-Yamaguchi, Nozomu Kira, Yuji Tomaru3, Takuma Okami, Takamichi Yoshimatsu, HOU Liyuan, OHAMA Takeshi, Kazunari Fukunaga, Masanori Okauchi5, Haruo Yamaguchi, Kohei Ohnishi, Angela Falciatore
    Title: Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum
    Journal: Scientific Reports, Vol. 5, No. 18708, pp. 1-13, Publishers of Nature
    Year: 2015
  12. Authors: Takashi Kadono, Nozomu Kira, Kengo Suzuki, Osamu Iwata, OHAMA Takeshi, Tomohiro Nishimura, Mai Akakabe, Masashi Tsuda, Masao Adachi
    Title: Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum
    Journal: Marine Drugs, Vol. 13, No. 8, pp. 5334-5357, MDPI
    Year: 2015
  13. Authors: KONG Fantao , Tomohito Yamasaki, Sari Dewi Kurniasih, HOU Liyuan , Xiaobo Li, Nina Ivanova, Shigeru Okada, Takeshi Ohama
    Title: Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains
    Journal: J. Bioscience and Bioengineering, Elsevjier
    Year: 2015
  14. Authors: HOU Liyuan , Hyunsun Park, Shigeru Okada, OHAMA Takeshi
    Title: Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments
    Journal: Protoplasma, Vol. 251, No. 1, pp. 191-199, Springer
    Year: 2014
  15. Authors: KONG Fantao, Yamasaki Tomohito, OHAMA Takeshi
    Title: Expression levels of domestic cDNA cassettes integrated in the nuclear genomes of various Chlamydomonas reinhardtii strains
    Journal: J. Biosc.Bioeng.
    Year: 2013
  16. Authors: HOU Liyuan, Wang Pengyu, KONG Fantao, PARKHyun-Sun, KOBIRO Kazuya, OHAMA Takeshi
    Title: Mesoporous TiO2 nanoparticles: a new material for biolistic bombardment
    Journal: Phycol. Res, Vol. 61, pp. 58-60
    Year: 2013
  17. Authors: UCHIDA Hidenobu, IKEUCHI ERI, YAMASAKI Tomohito, OHAMA Takeshi
    Title: The role of zinc finger protein in RNAi in a unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) in press
    Journal: J Phycology
    Year: 2012
  18. Authors: T. Yamasaki, T. Ohama
    Title: Involvement of Elongin C in the spread of repressive histone modifications
    Journal: The Plant J.
    Year: 2011
  19. Authors: Yamasaki T, Ohama T
    Title: Involvement of Elongin C in the spread of repressive histone modifications.
    Journal: The Plant Journal, Vol. 65, pp. 51-61
    Year: 2011
  20. Authors: Park HyunSun, OHAMA Takeshi
    Title: Biotransformation of a herb plant metabolite by a cell disruptant of Chlamydomonas reinhardtii
    Journal: Biosci. Biotechnol. Biochem., Vol. 12, pp. 2803-2805
    Year: 2009
  21. Authors: Ikuta K., Kawai H., Müler D.G., OHAMA Takeshi
    Title: Recurrent invasion of mitochondrial group II introns in specimens of Pylaiella littoralis (brown alga), collected worldwide
    Journal: Current Gent, Vol. 53, pp. 207-216
    Year: 2008
  22. Authors: OHAMA Takeshi, Inagaki Yuji, Bessho Yoshitaka, Osawa Syozo
    Title: Evolving genetic code
    Journal: Proceedings of the Japan Acad, Vol. 84, pp. 58-74
    Year: 2008
  23. Authors: Yamasaki T, Miyasaka H, Ohama T
    Title: Unstable RNAi effects through epigenetic silencing of an inverted repeat transgene in Chlamydomonas reinhardtii.
    Journal: Genetics., Vol. 180, pp. 1927-1944
    Year: 2008
  24. Authors: Yamasaki Tomohito, Miyasaka Hitoshi, OHAMA Takeshi
    Title: Unstable RNAi effect through methyl-CpG related transcriptional repression of inverted repeat transgene in Chlamydomonas reinhardtii.
    Journal: Genetics, Vol. 180, pp. 1927-1944
    Year: 2008
  25. Authors: Kurokawa S, Yamasaki T, Komatsu T, Watanabe KI, Ohama T
    Title: Degenerated recognition property of a mitochondrial homing enzyme in the unicellular green alga Chlamydomonas smithii.
    Journal: Plant Mol Biol., Vol. 62, pp. 141-150
    Year: 2006
  26. Authors: Kurokawa S, Tomohito Yamasaki, Komatsu T., Watanabw K., Ohama T
    Title: Degenerated recognition property of a mitochondrial Roming enzyme in the unicellular green alga chlamydomonas smithii
    Journal: Plant Molecular Biology, Vol. 62, pp. 141-150
    Year: 2006
  27. Authors: Yamasaki T, Kurokawa S., Watanabe K., Ikuta K., Ohama T
    Title: Shared moleculag charactrics of success fully tranoformed mitochondrial genomes in Chlamydomonas reinhaidtii
    Journal: Plant Mol. Biol, No. 58, pp. 515-527
    Year: 2005
  28. Authors: Kurokawa S, Bessho Y., Watanabe K, Ohama T
    Title: Adaptation of intronic homing endonuclease for successful hurizontal transmission
    Journal: FEBS J, No. 272, pp. 2487-2496
    Year: 2005
  29. Authors: Yamasaki T, Kurokawa S, Watanabe KI, Ikuta K, Ohama T
    Title: Shared molecular characteristics of successfully transformed mitochondrial genomes in Chlamydomonas reinhardtii.
    Journal: Plant Mol Biol., Vol. 58, pp. 515-527
    Year: 2005
  30. Authors: Watanabe I.K., Ohama T
    Title: Regular spliceosomal introns are invasive in Chlamydomonas reinhardtii : 15 introns in the recently relocated mitochondrial cox2 and cox3 genes
    Journal: J. Mol. Evol, Vol. 53, No. 4-5, pp. 333-339
    Year: 2001
  31. Authors: Inagaki Y., Dacks J., Doolittle W.F., Watanabe K., Ohama T
    Title: Evolutionary relationship between dinoflagellates bearing obligate diatom endosymbionts : insight into tertiary endosymbiosis
    Journal: Int. J. Sys. Evol. Microbiol, No. 50, pp. 2075-2081
    Year: 2000
  32. Authors: Ehara M, Inagaki Y., Watanabe I.K., Ohama T
    Title: Phylogenetic analysis of diatom coxI genes and implications of fluctuating GC content on mitochondrial genetic code evolution
    Journal: Current Genetics, No. 37, pp. 29-33
    Year: 2000
  33. Authors: Ehara M., Watanabe I.K., Ohama T
    Title: Distribution of cognates of group II introns detected in mitochondrial cox1 genes of a diatom and a haptophyte
    Journal: Gene, No. 256, pp. 157-167
    Year: 2000
  34. Authors: Hayashi-Ishimaru, Ehara M, Ohama T, Inagaki Y
    Title: Adeviant mitochondrial genetic code in prymnesiophytes (yellow-algae) : UGA codon for tryptophan.
    Journal: Current Genetics, No. 32, pp. 296-299
    Year: 1997
  35. Authors: Ohama T, Suzuki T., Mori M, Osawa S., Ueda T, Watanabe K, Nakase T
    Title: Non-univrsal decoding of the leucine codon CUG in ceveral Candida species.,
    Journal: Nucl. Acids Res., No. 21, pp. 4039-4045
    Year: 1993
  36. Authors: Jukes T. H, Bessho Y, Ohama T, Osawa S
    Title: Release factors and genetic code ( Scientific Correspondence)
    Journal: Nature, No. 352, p. 575
    Year: 1991
  37. Authors: Osawa S, Muto A, Ohama T, Andachi Y, Tanaka R, Yamano F
    Title: Procaryotic genetic code. Genetic code 1990 EXPERIENTIA
    Journal: No. 46, pp. 1097-1106
    Year: 1990
Keynote lectures
  1. Potential use of isobutyl-cyanoacrylate nanopartticle for inducing rapid cell wall disruption in Chlorella vurgaris , 18th INITERNATIONAL CONFERENCE ON THE CELL AND MOLECULAR BIOLOGY OF CHLAMYDOMONAS, Washington DC. USA, 2018
  2. The RNA-binding protein DUS16 plays an essential role in primary miRNA processing in Chlamydomnas , The international conference on the Cell and Molucular biology of Chlamydomas, Kyoto, Japan, 2016
Grants-in-Aid for Scientific Research from the Japanese government
  1. Project title: Analyses of abnormal physiological responses induced by stable resin nanoparticles and its species spectrum
    Category: Grant-in-Aid for Scientific Research (C)
    Project number: 19K12422
    Project period: 2019-2021
    Total budget amount: 4,290,000 yen

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Social activities

Committee roles
  1. Molecular Genetic Tools and Emerging Synthetic Biology Strategies to Increase Cellular Oil Content in Chlamydomonas reinhardtii, 2019

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