Faculty Members

LANGUAGE ≫ Japanese


  • Year of birth: 1965
  • Gender: Male
  • Affiliation:
    - School of Engineering Science
    - School of Environmental Science and Engineering
    - Life Science and Technology, Department of Engineering, Graduate School of Enginering
  • researchmap: https://researchmap.jp/setanaka
Areas of specialization Molecular Biology
Laboratory/research office Cell Proliferation Control Laboratory
Control of cell proliferation is the most fundamental and important aspect of life. When cells proliferate, chromosomal DNA, the blueprint of every organism, must be precisely replicated and segregated. Any defect in these processes can result in death or in diseases such as cancer. Our laboratory is conducting research using yeast, which is a model system of eukaryotic cells, to reveal the regulatory mechanism of cell cycles and chromosomal DNA replication, and further to understand the mechanism of stable genome maintenance.
Current research topics Regulation of chromosomal DNA replication in eukaryotes
Mechanisms for stable genome maintenance
Mechanisms for site-specific gene amplification
Interplay between the initiation of DNA replication and the regulation of chromatin
Educational background 1993: Doctor, Kobe University
1990: Master, Kobe University
1988: Bachelor, Kobe University
Professional background 2017-: Professor, Kochi University of Technology, School of Environmental Ecience and Engineering
2003-2017: Assistant Professor, National Institute of Genetics
1999-2003: Research Fellow in Imperial Cancer Research Fund - Cancer Research UK
1999-2001: JSPS Overseas Research Fellow
1994-2001: Assistant Professor, Osaka University, Research Institute of Microbial Diseases
1993-1994: JSPS Postdoctral Fellow
Academic societies The Molecular Biology Society of Japan, The Genetics Society of Japan, Yeast Genetics Society of Japan.

You can see the continuation of the table with a side swipe.


* Courses provided in English are shown with (E) mark

Undergraduate school
  • Molecular biology of cell proliferation regulation
  • Genetics
  • Biology Experiment
  • Graduation Thesis
  • Molecular Biology
Graduate school
  • Seminar 1
  • Seminar 2
  • Seminar 3
  • Seminar 4
  • Individual Work for Graduate
  • Advanced molecular biology
  • System biology
  • Advanced Seminar 2
  • Advanced Seminar 1

You can see the continuation of the table with a side swipe.

Research activities

Research papers
  1. Authors: Yuna Satake, Nozomi Gotoda, TANAKA SEIJI
    Title: Isolating Interaction-Null/Impaired Mutants using the Yeast Two-Hybrid Assay
    Journal: JoVE (Journal of Visualized Experiments), Vol. 202, p. e66423
    Year: 2023
  2. Authors: Seiji Tanaka, Shiho Ogawa
    Title: Dimerization of Firing Factors for Replication Origin Activation in Eukaryotes: A Crucial Process for Simultaneous Assembly of Bidirectional Replication Forks?
    Journal: Biology, Vol. 11, No. 6, p. 928, MDPI
    Year: 2022
  3. Authors: Seiji Tanaka
    Title: Interaction of replication factor Sld3 and histone acetyl transferase Esa1 alleviates gene silencing and promotes the activation of late and dormant replication origins
    Journal: Genetics, Vol. 217,iyaa001, The Genetics Society of America (GSA)
    Year: 2021
  4. Authors: Seiji Tanaka
    Title: Construction of Tight Conditional Mutants Using the Improved Auxin-Inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae
    Journal: Methods in Molecular Biology, Yeast Protocols, Vol. 2196, No. ISBN: 978-1-0716-0868-5, pp. 15-26, Springer
    Year: 2020
  5. Authors: Seiji Tanaka
    Title: An efficient method for the isolation of interaction‐null/impaired mutants using the yeast two‐hybrid technique
    Journal: Genes to Cells, Vol. 24, pp. 781-788, Wiley
    Year: 2019
  6. Authors: Riki Terui, Koji Nagao, Yoshitaka Kawasoe, Kanae Taki, Torahiko L. Higashi, Seiji Tanaka, Takuro Nakagawa, Chikashi Obuse, Hisao Masukata, Tatsuro S. Takahashi
    Title: Nucleosomes around a mismatched base pair are excluded via an Msh2-dependent reaction with the aid of SNF2 family ATPase Smarcad1
    Journal: GENES & DEVELOPMENT, Vol. 32, pp. 806-821, Cold Spring Harbor Laboratory Press
    Year: 2018
  7. Authors: M Miyazawa‐Onami, H Araki, Seiji Tanaka
    Title: Pre‐initiation complex assembly functions as a molecular switch that splits the Mcm2‐7 double hexamer
    Journal: EMBO reports, Vol. 18, No. 10, pp. 1752-1761, EMBO Press
    Year: 2017
  8. Authors: GH Goto, H Ogi, H Biswas, A Ghosh, Seiji Tanaka, K Sugimoto
    Title: Two separate pathways regulate protein stability of ATM/ATR-related protein kinases Mec1 and Tel1 in budding yeast
    Journal: PLoS Genetics, Vol. 13, No. 8, p. e1006873, Public Library of Science
    Year: 2017
  9. Authors: Seiji Tanaka, M Miyazawa‐Onami, T Iida, H Araki
    Title: iAID: an improved auxin‐inducible degron system for the construction of a ‘tight’conditional mutant in the budding yeast Saccharomyces cerevisiae
    Journal: Yeast, Vol. 32, No. 8, pp. 567-581
    Year: 2015
  10. Authors: Seiji Tanaka, Y Komeda, T Umemori, Y Kubota, H Takisawa, H Araki
    Title: Efficient initiation of DNA replication in eukaryotes requires Dpb11/TopBP1-GINS interaction
    Journal: Molecular and Cellular Biology, Vol. 33, No. 13, pp. 2614-2622, American Society for Microbiology
    Year: 2013
  11. Authors: Seiji Tanaka, R Nakato, Y Katou, K Shirahige, H Araki
    Title: Origin association of Sld3, Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing
    Journal: Current Biology, Vol. 21, No. 24, pp. 2055-2063, Cell Press
    Year: 2011
  12. Authors: Seiji Tanaka, H Araki
    Title: Multiple regulatory mechanisms to inhibit untimely initiation of DNA replication are important for stable genome maintenance
    Journal: PLoS Genetics, Vol. 7, No. 6, p. e1002136, Public Library of Science
    Year: 2011
  13. Authors: Seiji Tanaka, T Umemori, K Hirai, S Muramatsu, Y Kamimura, H Araki
    Title: CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast
    Journal: Nature, Vol. 445, No. 7125, pp. 328-332, Nature Publishing Group
    Year: 2007
  14. Authors: Yoshikazu Ohya, Seiji Tanaka, et al.
    Title: High-dimensional and large-scale phenotyping of yeast mutants.
    Journal: Proc Natl Acad Sci USA, Vol. 102, No. 52, pp. 19015-19020
    Year: 2005
  15. Authors: S. Mimura, T. Seki, S. Tanaka, J. F. X. Diffley
    Title: Phosphorylation-dependent binding of mitotic cyclins to Cdc6 contributes to DNA replication control
    Journal: Nature, Vol. 431, pp. 1118-1123
    Year: 2004
  16. Authors: Seiji Tanaka, JFX Diffley
    Title: Deregulated G1-cyclin expression induces genomic instability by preventing efficient pre-RC formation
    Journal: Genes & Development , Vol. 16, No. 20, pp. 2639-2649, Cold Spring Harbor Lab
    Year: 2002
  17. Authors: Seiji Tanaka, JFX Diffley
    Title: Interdependent nuclear accumulation of budding yeast Cdt1 and Mcm2-7 during G1 phase
    Journal: Nature Cell Biology, Vol. 4, No. 3, pp. 198-207, Nature Publishing Group
    Year: 2002
Invited lectures
  1. Identification of atypical replication origin in the metallothionein-encoding repeats in the budding yeast Saccharomyces cerevisiae, NIG International Symposium, 2022

You can see the continuation of the table with a side swipe.

Social activities

Published books
  1. Role of CDK in Replication Initiation (chapter 13 in the book: The Initiation of DNA Replication in Eukaryotes), Springer International Publishing, 2016, ISBN 978-3-319-246

You can see the continuation of the table with a side swipe.